The CamSol Method Web Servers

(more info on the method)

  • Quantitative solubility ranking of protein variants (sequence-based).

  • Identification of aggregation hotspots on protein structure.

  • Rational design of protein variants with enhanced solubility. 

 

Free for academic users (registration/login required). Commercial users please contact Professor Michele Vendruscolo.

CamSol Intrinsic Sequence-based method of predicting protein solubility and generic aggregation propensity.
CamSol Structurally Corrected Structure-based method of calculating a solubility profile, which accounts for the proximity of the amino acids in the structure and for their solvent exposure. The structurally corrected profile can be color-coded on the structure of the protein to spot patches of low solubility that may elicit self-assembly.
CamSol design A method of rational design of protein variants with enhanced solubility (more info on the CamSol method)

 

References:

P. Sormanni, F. A. Aprile and M. Vendruscolo. 
The CamSol Method of Rational Design of Protein Mutants with Enhanced Solubility. 
J. Mol. Biol. 2015. doi:10.1016/j.jmb.2014.09.026.

P. Sormanni, L. Amery, S. Ekizoglou, M. Vendruscolo, and B. Popovic.
Rapid and accurate in silico solubility screening of a monoclonal antibody library. 
Sci. Rep.  2017. doi:10.1038/s41598-017-07800-w.